RNAi-mediated downregulation of poplar plasma membrane intrinsic proteins (PIPs) changes plasma membrane proteome composition and affects leaf physiology.
Identifieur interne : 001B65 ( Main/Exploration ); précédent : 001B64; suivant : 001B66RNAi-mediated downregulation of poplar plasma membrane intrinsic proteins (PIPs) changes plasma membrane proteome composition and affects leaf physiology.
Auteurs : Zhen Bi [Allemagne] ; Juliane Merl-Pham [Allemagne] ; Norbert Uehlein [Allemagne] ; Ina Zimmer [Allemagne] ; Stefanie Mühlhans [Allemagne] ; Michaela Aichler [Allemagne] ; Axel Karl Walch [Allemagne] ; Ralf Kaldenhoff [Allemagne] ; Klaus Palme [Allemagne] ; Jörg-Peter Schnitzler [Allemagne] ; Katja Block [Allemagne]Source :
- Journal of proteomics [ 1876-7737 ] ; 2015.
Descripteurs français
- KwdFr :
- Aquaporines (métabolisme), Extinction de l'expression des gènes (physiologie), Feuilles de plante (MeSH), Interférence par ARN (physiologie), Populus (physiologie), Protéines végétales (métabolisme), Protéome (métabolisme), Régulation négative (physiologie), Transpiration des plantes (physiologie), Végétaux génétiquement modifiés (physiologie).
- MESH :
English descriptors
- KwdEn :
- MESH :
- chemical , metabolism : Aquaporins, Plant Proteins, Proteome.
- physiology : Down-Regulation, Gene Silencing, Plant Transpiration, Plants, Genetically Modified, Populus, RNA Interference.
- Plant Leaves.
Abstract
Plasma membrane intrinsic proteins (PIPs) are one subfamily of aquaporins that mediate the transmembrane transport of water. To reveal their function in poplar, we generated transgenic poplar plants in which the translation of PIP genes was downregulated by RNA interference investigated these plants with a comprehensive leaf plasma membrane proteome and physiome analysis. First, inhibition of PIP synthesis strongly altered the leaf plasma membrane protein composition. Strikingly, several signaling components and transporters involved in the regulation of stomatal movement were differentially regulated in transgenic poplars. Furthermore, hormonal crosstalk related to abscisic acid, auxin and brassinosteroids was altered, in addition to cell wall biosynthesis/cutinization, the organization of cellular structures and membrane trafficking. A physiological analysis confirmed the proteomic results. The leaves had wider opened stomata and higher net CO2 assimilation and transpiration rates as well as greater mesophyll conductance for CO2 (gm) and leaf hydraulic conductance (Kleaf). Based on these results, we conclude that PIP proteins not only play essential roles in whole leaf water and CO2 flux but have important roles in the regulation of stomatal movement.
DOI: 10.1016/j.jprot.2015.07.029
PubMed: 26248320
Affiliations:
- Allemagne
- Bade-Wurtemberg, District de Darmstadt, District de Fribourg-en-Brisgau, Hesse (Land)
- Darmstadt, Fribourg-en-Brisgau
Links toward previous steps (curation, corpus...)
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Neuherberg</wicri:noRegion></affiliation></author><author><name sortKey="Merl Pham, Juliane" sort="Merl Pham, Juliane" uniqKey="Merl Pham J" first="Juliane" last="Merl-Pham">Juliane Merl-Pham</name><affiliation wicri:level="1"><nlm:affiliation>Research Unit Protein Science-Core Facility Proteomics, Helmholtz Zentrum München, Ingolstädter Landstr.1, 85764 Neuherberg, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Research Unit Protein Science-Core Facility Proteomics, Helmholtz Zentrum München, Ingolstädter Landstr.1, 85764 Neuherberg</wicri:regionArea><wicri:noRegion>85764 Neuherberg</wicri:noRegion><wicri:noRegion>85764 Neuherberg</wicri:noRegion><wicri:noRegion>85764 Neuherberg</wicri:noRegion></affiliation></author><author><name sortKey="Uehlein, Norbert" sort="Uehlein, Norbert" uniqKey="Uehlein N" first="Norbert" last="Uehlein">Norbert Uehlein</name><affiliation wicri:level="3"><nlm:affiliation>Institute of Applied Plant Science, University of Technology Darmstadt, Schnittspahndtr.10, 64287 Darmstadt, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institute of Applied Plant Science, University of Technology Darmstadt, Schnittspahndtr.10, 64287 Darmstadt</wicri:regionArea><placeName><region type="land" nuts="1">Hesse (Land)</region><region type="district" nuts="2">District de Darmstadt</region><settlement type="city">Darmstadt</settlement></placeName></affiliation></author><author><name sortKey="Zimmer, Ina" sort="Zimmer, Ina" uniqKey="Zimmer I" first="Ina" last="Zimmer">Ina Zimmer</name><affiliation wicri:level="1"><nlm:affiliation>Research Unit Environmental Simulation, Institute of Biochemical Plant Pathology, Helmholtz Zentrum München, Ingolstädter Landstr.1, 85764 Neuherberg, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Research Unit Environmental Simulation, Institute of Biochemical Plant Pathology, Helmholtz Zentrum München, Ingolstädter 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sortKey="Aichler, Michaela" sort="Aichler, Michaela" uniqKey="Aichler M" first="Michaela" last="Aichler">Michaela Aichler</name><affiliation wicri:level="1"><nlm:affiliation>Research Unit Analytical Pathology, Helmholtz Zentrum München, Ingolstädter Landstr.1, 85764 Neuherberg, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Research Unit Analytical Pathology, Helmholtz Zentrum München, Ingolstädter Landstr.1, 85764 Neuherberg</wicri:regionArea><wicri:noRegion>85764 Neuherberg</wicri:noRegion><wicri:noRegion>85764 Neuherberg</wicri:noRegion><wicri:noRegion>85764 Neuherberg</wicri:noRegion></affiliation></author><author><name sortKey="Walch, Axel Karl" sort="Walch, Axel Karl" uniqKey="Walch A" first="Axel Karl" last="Walch">Axel Karl Walch</name><affiliation wicri:level="1"><nlm:affiliation>Research Unit Analytical Pathology, Helmholtz Zentrum München, Ingolstädter Landstr.1, 85764 Neuherberg, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Research Unit Analytical Pathology, Helmholtz Zentrum München, Ingolstädter Landstr.1, 85764 Neuherberg</wicri:regionArea><wicri:noRegion>85764 Neuherberg</wicri:noRegion><wicri:noRegion>85764 Neuherberg</wicri:noRegion><wicri:noRegion>85764 Neuherberg</wicri:noRegion></affiliation></author><author><name sortKey="Kaldenhoff, Ralf" sort="Kaldenhoff, Ralf" uniqKey="Kaldenhoff R" first="Ralf" last="Kaldenhoff">Ralf Kaldenhoff</name><affiliation wicri:level="3"><nlm:affiliation>Institute of Applied Plant Science, University of Technology Darmstadt, Schnittspahndtr.10, 64287 Darmstadt, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institute of Applied Plant Science, University of Technology Darmstadt, Schnittspahndtr.10, 64287 Darmstadt</wicri:regionArea><placeName><region type="land" nuts="1">Hesse (Land)</region><region type="district" nuts="2">District de Darmstadt</region><settlement type="city">Darmstadt</settlement></placeName></affiliation></author><author><name sortKey="Palme, Klaus" sort="Palme, Klaus" uniqKey="Palme K" first="Klaus" last="Palme">Klaus Palme</name><affiliation wicri:level="3"><nlm:affiliation>BIOSS Centre for Biological Signalling Studies, ZBSA Centre for Biosystems Studies, Faculty of Biology, Schänzlestr. 1, University of Freiburg, 79104 Freiburg, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>BIOSS Centre for Biological Signalling Studies, ZBSA Centre for Biosystems Studies, Faculty of Biology, Schänzlestr. 1, University of Freiburg, 79104 Freiburg</wicri:regionArea><placeName><region type="land" nuts="1">Bade-Wurtemberg</region><region type="district" nuts="2">District de Fribourg-en-Brisgau</region><settlement type="city">Fribourg-en-Brisgau</settlement></placeName></affiliation></author><author><name sortKey="Schnitzler, Jorg Peter" sort="Schnitzler, Jorg Peter" uniqKey="Schnitzler J" first="Jörg-Peter" last="Schnitzler">Jörg-Peter Schnitzler</name><affiliation wicri:level="1"><nlm:affiliation>Research Unit Environmental Simulation, Institute of Biochemical Plant Pathology, Helmholtz Zentrum München, Ingolstädter Landstr.1, 85764 Neuherberg, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Research Unit Environmental Simulation, Institute of Biochemical Plant Pathology, Helmholtz Zentrum München, Ingolstädter Landstr.1, 85764 Neuherberg</wicri:regionArea><wicri:noRegion>85764 Neuherberg</wicri:noRegion><wicri:noRegion>85764 Neuherberg</wicri:noRegion><wicri:noRegion>85764 Neuherberg</wicri:noRegion></affiliation></author><author><name sortKey="Block, Katja" sort="Block, Katja" uniqKey="Block K" first="Katja" last="Block">Katja Block</name><affiliation wicri:level="1"><nlm:affiliation>Research Unit Environmental Simulation, Institute of Biochemical Plant Pathology, Helmholtz Zentrum München, Ingolstädter Landstr.1, 85764 Neuherberg, Germany. Electronic address: katja.block@helmholtz-muenchen.de.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Research Unit Environmental Simulation, Institute of Biochemical Plant Pathology, Helmholtz Zentrum München, Ingolstädter Landstr.1, 85764 Neuherberg</wicri:regionArea><wicri:noRegion>85764 Neuherberg</wicri:noRegion><wicri:noRegion>85764 Neuherberg</wicri:noRegion><wicri:noRegion>85764 Neuherberg</wicri:noRegion></affiliation></author></analytic><series><title level="j">Journal of proteomics</title><idno type="eISSN">1876-7737</idno><imprint><date when="2015" type="published">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Aquaporins (metabolism)</term><term>Down-Regulation (physiology)</term><term>Gene Silencing (physiology)</term><term>Plant Leaves (MeSH)</term><term>Plant Proteins (metabolism)</term><term>Plant Transpiration (physiology)</term><term>Plants, Genetically Modified (physiology)</term><term>Populus (physiology)</term><term>Proteome (metabolism)</term><term>RNA Interference (physiology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Aquaporines (métabolisme)</term><term>Extinction de l'expression des gènes (physiologie)</term><term>Feuilles de plante (MeSH)</term><term>Interférence par ARN (physiologie)</term><term>Populus (physiologie)</term><term>Protéines végétales (métabolisme)</term><term>Protéome (métabolisme)</term><term>Régulation négative (physiologie)</term><term>Transpiration des plantes (physiologie)</term><term>Végétaux génétiquement modifiés (physiologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Aquaporins</term><term>Plant Proteins</term><term>Proteome</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Aquaporines</term><term>Protéines végétales</term><term>Protéome</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Extinction de l'expression des gènes</term><term>Interférence par ARN</term><term>Populus</term><term>Régulation négative</term><term>Transpiration des plantes</term><term>Végétaux génétiquement modifiés</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Down-Regulation</term><term>Gene Silencing</term><term>Plant Transpiration</term><term>Plants, Genetically Modified</term><term>Populus</term><term>RNA Interference</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Plant Leaves</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Feuilles de plante</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Plasma membrane intrinsic proteins (PIPs) are one subfamily of aquaporins that mediate the transmembrane transport of water. To reveal their function in poplar, we generated transgenic poplar plants in which the translation of PIP genes was downregulated by RNA interference investigated these plants with a comprehensive leaf plasma membrane proteome and physiome analysis. First, inhibition of PIP synthesis strongly altered the leaf plasma membrane protein composition. Strikingly, several signaling components and transporters involved in the regulation of stomatal movement were differentially regulated in transgenic poplars. Furthermore, hormonal crosstalk related to abscisic acid, auxin and brassinosteroids was altered, in addition to cell wall biosynthesis/cutinization, the organization of cellular structures and membrane trafficking. A physiological analysis confirmed the proteomic results. The leaves had wider opened stomata and higher net CO2 assimilation and transpiration rates as well as greater mesophyll conductance for CO2 (gm) and leaf hydraulic conductance (Kleaf). Based on these results, we conclude that PIP proteins not only play essential roles in whole leaf water and CO2 flux but have important roles in the regulation of stomatal movement. </div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">26248320</PMID><DateCompleted><Year>2016</Year><Month>09</Month><Day>02</Day></DateCompleted><DateRevised><Year>2015</Year><Month>10</Month><Day>23</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1876-7737</ISSN><JournalIssue CitedMedium="Internet"><Volume>128</Volume><PubDate><Year>2015</Year><Month>Oct</Month><Day>14</Day></PubDate></JournalIssue><Title>Journal of proteomics</Title><ISOAbbreviation>J Proteomics</ISOAbbreviation></Journal><ArticleTitle>RNAi-mediated downregulation of poplar plasma membrane intrinsic proteins (PIPs) changes plasma membrane proteome composition and affects leaf physiology.</ArticleTitle><Pagination><MedlinePgn>321-32</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.jprot.2015.07.029</ELocationID><ELocationID EIdType="pii" ValidYN="Y">S1874-3919(15)30082-8</ELocationID><Abstract><AbstractText>Plasma membrane intrinsic proteins (PIPs) are one subfamily of aquaporins that mediate the transmembrane transport of water. To reveal their function in poplar, we generated transgenic poplar plants in which the translation of PIP genes was downregulated by RNA interference investigated these plants with a comprehensive leaf plasma membrane proteome and physiome analysis. First, inhibition of PIP synthesis strongly altered the leaf plasma membrane protein composition. Strikingly, several signaling components and transporters involved in the regulation of stomatal movement were differentially regulated in transgenic poplars. Furthermore, hormonal crosstalk related to abscisic acid, auxin and brassinosteroids was altered, in addition to cell wall biosynthesis/cutinization, the organization of cellular structures and membrane trafficking. A physiological analysis confirmed the proteomic results. The leaves had wider opened stomata and higher net CO2 assimilation and transpiration rates as well as greater mesophyll conductance for CO2 (gm) and leaf hydraulic conductance (Kleaf). Based on these results, we conclude that PIP proteins not only play essential roles in whole leaf water and CO2 flux but have important roles in the regulation of stomatal movement. </AbstractText><CopyrightInformation>Copyright © 2015. Published by Elsevier B.V.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Bi</LastName><ForeName>Zhen</ForeName><Initials>Z</Initials><AffiliationInfo><Affiliation>Research Unit Environmental Simulation, Institute of Biochemical Plant Pathology, Helmholtz Zentrum München, Ingolstädter Landstr.1, 85764 Neuherberg, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Merl-Pham</LastName><ForeName>Juliane</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Research Unit Protein Science-Core Facility Proteomics, Helmholtz Zentrum München, Ingolstädter Landstr.1, 85764 Neuherberg, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Uehlein</LastName><ForeName>Norbert</ForeName><Initials>N</Initials><AffiliationInfo><Affiliation>Institute of Applied Plant Science, University of Technology Darmstadt, Schnittspahndtr.10, 64287 Darmstadt, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zimmer</LastName><ForeName>Ina</ForeName><Initials>I</Initials><AffiliationInfo><Affiliation>Research Unit Environmental Simulation, Institute of Biochemical Plant Pathology, Helmholtz Zentrum München, Ingolstädter Landstr.1, 85764 Neuherberg, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Mühlhans</LastName><ForeName>Stefanie</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Research Unit Environmental Simulation, Institute of Biochemical Plant Pathology, Helmholtz Zentrum München, Ingolstädter Landstr.1, 85764 Neuherberg, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Aichler</LastName><ForeName>Michaela</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Research Unit Analytical Pathology, Helmholtz Zentrum München, Ingolstädter Landstr.1, 85764 Neuherberg, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Walch</LastName><ForeName>Axel Karl</ForeName><Initials>AK</Initials><AffiliationInfo><Affiliation>Research Unit Analytical Pathology, Helmholtz Zentrum München, Ingolstädter Landstr.1, 85764 Neuherberg, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kaldenhoff</LastName><ForeName>Ralf</ForeName><Initials>R</Initials><AffiliationInfo><Affiliation>Institute of Applied Plant Science, University of Technology Darmstadt, Schnittspahndtr.10, 64287 Darmstadt, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Palme</LastName><ForeName>Klaus</ForeName><Initials>K</Initials><AffiliationInfo><Affiliation>BIOSS Centre for Biological Signalling Studies, ZBSA Centre for Biosystems Studies, Faculty of Biology, Schänzlestr. 1, University of Freiburg, 79104 Freiburg, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Schnitzler</LastName><ForeName>Jörg-Peter</ForeName><Initials>JP</Initials><AffiliationInfo><Affiliation>Research Unit Environmental Simulation, Institute of Biochemical Plant Pathology, Helmholtz Zentrum München, Ingolstädter Landstr.1, 85764 Neuherberg, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Block</LastName><ForeName>Katja</ForeName><Initials>K</Initials><AffiliationInfo><Affiliation>Research Unit Environmental Simulation, Institute of Biochemical Plant Pathology, Helmholtz Zentrum München, Ingolstädter Landstr.1, 85764 Neuherberg, Germany. Electronic address: katja.block@helmholtz-muenchen.de.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2015</Year><Month>08</Month><Day>04</Day></ArticleDate></Article><MedlineJournalInfo><Country>Netherlands</Country><MedlineTA>J Proteomics</MedlineTA><NlmUniqueID>101475056</NlmUniqueID><ISSNLinking>1874-3919</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D020346">Aquaporins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010940">Plant Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D020543">Proteome</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="C110065">major intrinsic protein, plant</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D020346" MajorTopicYN="N">Aquaporins</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015536" MajorTopicYN="N">Down-Regulation</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D020868" MajorTopicYN="N">Gene Silencing</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018515" MajorTopicYN="N">Plant Leaves</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010940" MajorTopicYN="N">Plant Proteins</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018526" MajorTopicYN="N">Plant Transpiration</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D030821" MajorTopicYN="N">Plants, Genetically Modified</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D020543" MajorTopicYN="N">Proteome</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D034622" MajorTopicYN="N">RNA Interference</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Aquaporin</Keyword><Keyword MajorTopicYN="N">PIP</Keyword><Keyword MajorTopicYN="N">Photosynthesis</Keyword><Keyword MajorTopicYN="N">Plasma membrane proteomics</Keyword><Keyword MajorTopicYN="N">Populus×canescens</Keyword><Keyword MajorTopicYN="N">Stomatal movement</Keyword><Keyword MajorTopicYN="N">plasma membrane intrinsic protein</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2015</Year><Month>05</Month><Day>29</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2015</Year><Month>07</Month><Day>16</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2015</Year><Month>07</Month><Day>23</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2015</Year><Month>8</Month><Day>7</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2015</Year><Month>8</Month><Day>8</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2016</Year><Month>9</Month><Day>3</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">26248320</ArticleId><ArticleId IdType="pii">S1874-3919(15)30082-8</ArticleId><ArticleId IdType="doi">10.1016/j.jprot.2015.07.029</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Allemagne</li></country><region><li>Bade-Wurtemberg</li><li>District de Darmstadt</li><li>District de Fribourg-en-Brisgau</li><li>Hesse (Land)</li></region><settlement><li>Darmstadt</li><li>Fribourg-en-Brisgau</li></settlement></list><tree><country name="Allemagne"><noRegion><name sortKey="Bi, Zhen" sort="Bi, Zhen" uniqKey="Bi Z" first="Zhen" last="Bi">Zhen Bi</name></noRegion><name sortKey="Aichler, Michaela" sort="Aichler, Michaela" uniqKey="Aichler M" first="Michaela" last="Aichler">Michaela Aichler</name><name sortKey="Block, Katja" sort="Block, Katja" uniqKey="Block K" first="Katja" last="Block">Katja Block</name><name sortKey="Kaldenhoff, Ralf" sort="Kaldenhoff, Ralf" uniqKey="Kaldenhoff R" first="Ralf" last="Kaldenhoff">Ralf Kaldenhoff</name><name sortKey="Merl Pham, Juliane" sort="Merl Pham, Juliane" uniqKey="Merl Pham J" first="Juliane" last="Merl-Pham">Juliane Merl-Pham</name><name sortKey="Muhlhans, Stefanie" sort="Muhlhans, Stefanie" uniqKey="Muhlhans S" first="Stefanie" last="Mühlhans">Stefanie Mühlhans</name><name sortKey="Palme, Klaus" sort="Palme, Klaus" uniqKey="Palme K" first="Klaus" last="Palme">Klaus Palme</name><name sortKey="Schnitzler, Jorg Peter" sort="Schnitzler, Jorg Peter" uniqKey="Schnitzler J" first="Jörg-Peter" last="Schnitzler">Jörg-Peter Schnitzler</name><name sortKey="Uehlein, Norbert" sort="Uehlein, Norbert" uniqKey="Uehlein N" first="Norbert" last="Uehlein">Norbert Uehlein</name><name sortKey="Walch, Axel Karl" sort="Walch, Axel Karl" uniqKey="Walch A" first="Axel Karl" last="Walch">Axel Karl Walch</name><name 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